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primary bronchial epithelial cell culture primary bronchial  (ATCC)
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ATCC primary bronchial epithelial cell culture primary bronchial
Primary Bronchial Epithelial Cell Culture Primary Bronchial, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htbe cells  (ATCC)
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ATCC htbe cells
The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) <t>HTBE</t> <t>cells</t> were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .
Htbe Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bronchial tracheal epithelial cells  (ATCC)
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ATCC bronchial tracheal epithelial cells
The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) <t>HTBE</t> <t>cells</t> were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .
Bronchial Tracheal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcs  (ATCC)
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ATCC pcs
The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) <t>HTBE</t> <t>cells</t> were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .
Pcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a549 cells  (ATCC)
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ATCC a549 cells
Cytotoxicity of peptides 1–5 on <t>A549</t> cells detected by MTT assay. Cells were treated with 100 μM peptides 1–5 for 48 h. Results are presented as mean ± standard deviation, n = 3.
A549 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human bronchial epithelial cell line hbe  (ATCC)
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ATCC human bronchial epithelial cell line hbe
Cytotoxicity of peptides 1–5 on <t>A549</t> cells detected by MTT assay. Cells were treated with 100 μM peptides 1–5 for 48 h. Results are presented as mean ± standard deviation, n = 3.
Human Bronchial Epithelial Cell Line Hbe, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary hbec  (ATCC)
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ATCC primary hbec
Cytotoxicity of peptides 1–5 on <t>A549</t> cells detected by MTT assay. Cells were treated with 100 μM peptides 1–5 for 48 h. Results are presented as mean ± standard deviation, n = 3.
Primary Hbec, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human bronchoalveolar epithelial  (ATCC)
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ATCC normal human bronchoalveolar epithelial
Cytotoxicity of peptides 1–5 on <t>A549</t> cells detected by MTT assay. Cells were treated with 100 μM peptides 1–5 for 48 h. Results are presented as mean ± standard deviation, n = 3.
Normal Human Bronchoalveolar Epithelial, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) HTBE cells were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .

Journal: bioRxiv

Article Title: Small molecule inhibition of the mitochondrial lipid transfer protein STARD7 attenuates influenza viral replication

doi: 10.64898/2026.01.20.700505

Figure Lengend Snippet: The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) HTBE cells were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .

Article Snippet: HTBE cells (ATCC PCS-300-010) were cultured in commercially available airway epithelial cell basal media following the manufacturer’s protocol (ATCC).

Techniques: Virus, Infection, Staining, Immunofluorescence, Plaque Assay

Cytotoxicity of peptides 1–5 on A549 cells detected by MTT assay. Cells were treated with 100 μM peptides 1–5 for 48 h. Results are presented as mean ± standard deviation, n = 3.

Journal: Frontiers in Pharmacology

Article Title: Identification of a novel dual-targeting peptide inhibitor of SARS-CoV-2 RBD and NRP1 through structure-based virtual screening

doi: 10.3389/fphar.2025.1691679

Figure Lengend Snippet: Cytotoxicity of peptides 1–5 on A549 cells detected by MTT assay. Cells were treated with 100 μM peptides 1–5 for 48 h. Results are presented as mean ± standard deviation, n = 3.

Article Snippet: A549 cells (NHBE, catalog number: PCS-300-010) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States).

Techniques: MTT Assay, Standard Deviation